The effect of quercetin on topotecan cytotoxicity in MCF-7 and MDA-MB 231 human breast cancer cells

Akbas S. H., Timur M., Ozben T.

JOURNAL OF SURGICAL RESEARCH, cilt.125, sa.1, ss.49-55, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 125 Sayı: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.jss.2004.11.011
  • Dergi Adı: JOURNAL OF SURGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.49-55
  • Anahtar Kelimeler: breast cancer, Topotecan, ROS, nitrite, Quercetin, ESTROGEN-RECEPTOR, P-GLYCOPROTEIN, DRUG, PROLIFERATION, APOPTOSIS, ASSAY, INHIBITION, EXPRESSION, FLAVONOIDS, PEROXIDE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background. Topotecan, which is a Camptothecin derivative, shows a large spectrum in anti-tumor activity. Topotecan exerts its cytotoxic effect on tumor cells mainly by inhibition of topoisomerase I activity resulting in double-strand DNA breaks. In our study, we investigated the combined cytotoxic action of Topotecan and Quercetin in MCF-7 and MDA-MB 231 human breast cancer cells. To examine the possible relation between the cytotoxic activity of Topotecan and oxidative stress, we measured ROS and nitrite levels in both human breast cell lines.