A rapid molecular identification method for Meloidogyne chitwoodi from potato tubers without using nematode extraction methods

Uysal G., DEVRAN Z.

Journal of Phytopathology, cilt.170, sa.11-12, ss.753-757, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170 Sayı: 11-12
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1111/jph.13146
  • Dergi Adı: Journal of Phytopathology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.753-757
  • Anahtar Kelimeler: Columbia root-knot nematode, DNA isolation, molecular method, PCR
  • Akdeniz Üniversitesi Adresli: Evet

Özet

© 2022 Wiley-VCH GmbH.Potato (Solanum tuberosum L.) is one of the major crops for human nutrition. Columbia root-knot nematode, Meloidogyne chitwoodi Golden et al. (1980), can cause significant decrease in potato yields in the world. The aim of the study was to develop a novel molecular method for the rapid and accurate detection of M. chitwoodi on infested potato tubers. Total DNA from peel and tissues of potato were directly isolated from M. chitwoodi-infested potato tubers without using any nematode extraction method. Meloidogyne chitwoodi was successfully detected and identified in total DNA isolated using species-specific primers. Meloidogyne chitwoodi was also successfully identified in DNA isolated from only one necrotic spot in potato. Our results show that this molecular method is quite useful for fast and accurate identification of M. chitwoodi on potato. Based on these results, it seems that useful practical and applicable aspects for direct detection of M. chitwoodi from potato tubers without nematode extraction. The novel technique can be used in quarantine laboratories and potato seed producer companies. To the best of the authors' knowledge, this is the first report to identify M. chitwoodi using a novel molecular method from potato tubers without nematode extraction.