beta-Mannanase production and kinetic modeling from carob extract by using recombinant Aspergillus sojae

Karahalil E., Germec M., TURHAN İ.

BIOTECHNOLOGY PROGRESS, cilt.35, sa.6, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/btpr.2885
  • Dergi Adı: BIOTECHNOLOGY PROGRESS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: carob extract, fungal enzyme production, kinetic modeling, optimization, whey, ETHANOL-PRODUCTION, FERMENTATION, ENDO-BETA-1,4-MANNANASE, PERFORMANCE, FUMIGATUS, STRAIN, GROWTH
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The main objectives of this study were to optimize beta-mannanase fermentation conditions by using Response Surface Methodology (RSM) and to model kinetically using the kinetic models. Based on the results, the optimum fermentation conditions were found to be initial sugar concentration of 10 degrees Bx, whey concentration of 0.75% [w/v], and inoculum size of 8% (v/v). Under optimized conditions, beta-mannanase activity (P), sugar consumed (Delta S), maximum beta-mannanase production rate (Q(P)), and sugar utilization yield (SUY) were 687.89 U/mL, 47.38 g/L, 118.54 U mL(-1) day(-1), and 69.73%, respectively. Kinetic models were employed to describe the optimum beta-mannanase fermentation process. The kinetic analysis of beta-mannanase fermentation showed that beta-mannanase fermentation is growth associated because the alpha value (U/mgX) is approximately 330-fold higher than the beta value (U/mgX center dot hr). Nevertheless, maintenance value (Z) was lower than gamma value, thus showing that Aspergillus niger mainly utilizes the sugars for beta-mannanase production and fungal growth. Consequently, carob extract and whey powder could be used to be cost-effective carbon and organic nitrogen sources, respectively. It was clearly indicated that the suggested kinetic models can successfully describe the fungal growth, beta-mannanase production, and substrate consumption.