Akademik Veri Yönetim Sistemi
41st Federation of European Biochemical Societies (FEBS) congress, Aydın, Türkiye, 3 - 08 Eylül 2016, cilt.Suppl. 1, ss.394
The aim of our work was to compare prooxidant and antioxidant
properties of linalool, which is the oxygenated monoterpene compound
reported to be a major volatile component of the essential
oil of several aromatic species, in Hep G2 cells.
Cytotoxicity of linalool was assayed by CellTiter-Blue Cell
Viability Assay. Malondialdehyde levels result in membrane damage
in Hep G2 cells were assayed by using fluorometric method.
Hep G2 cells were incubated with linalool at 24, 48 and
72 hours. The viability of Hep G2 cells decreased in a manner
dependent upon concentration and incubation time. The IC50 values
were calculated 81.5 lg/ml (24 hours), 72.7 lg/ml (48 hours)
and 64.7 lg/ml (72 hours). But, cell viability of Hep G2 cells
increased when the cells preincubated with linalool at lower concentrations
(?IC50) against H2O2 cytotoxicity. Membrane-damaging
effects of linalool were increased with accelerating
concentrations. On the other hand, membrane damaging effect of
H2O2 was decreased when the cells preincubated with linalool
before H2O2 incubation.
Oxygenated monoterpene linalool had both prooxidant and
antioxidant properties showing membrane damaging and protective
effects on Hep G2 cells depend on concentration.