The distribution of transglutaminase in the rat oocytes and embryos

Akkoyunlu G., Uestuenel I., Demir R.

THERIOGENOLOGY, cilt.68, sa.6, ss.834-841, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 6
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.theriogenology.2007.06.015
  • Dergi Adı: THERIOGENOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.834-841
  • Anahtar Kelimeler: transglutarninase, fertilization, zona pellucida, cortical granules, rat, MAMMALIAN CORTICAL GRANULES, CHYMOTRYPSIN-LIKE PROTEASE, MOUSE ZONA-PELLUCIDA, XENOPUS-LAEVIS EGGS, TISSUE TRANSGLUTAMINASE, CROSS-LINKING, FERTILIZATION ENVELOPE, INVITRO FERTILIZATION, EXTRACELLULAR-MATRIX, PERIVITELLINE SPACE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Transglutaminases (TGs) are calcium-dependent enzymes that catalyze the transamidation of glutamine residues of a protein substrate to form intermolecular isopeptide bonds. The zona pellucida (ZP) is an extracellular, glycoprotein matrix that surrounds the oocytes of all Eutherian mammals. We aimed to identify the immunoreactivity of tissue transglutaminase (tTG) and ultrastructural changes occuring in rat oocytes before and after fertilization. Female rats were stimulated to superovulate, then mated with males. Oocytes and embryos were collected and examined by immunohistochemistry and electron microscopy. Before fertilization, tTG was present only in the oolemma and the cortical cytoplasm. After fertilization, tTG reactivity increased in the ZP of the early zygote and the preimplantation embryos, but decreased in the cytoplasm and perivitelline space (PVS). After fertilization, the PVS ultrastructure became asymmetrical and large around the polar bodies with many cortical granule contents. In conclusion, tTG immunoreactivity was found to be spatially and temporarily heterogeneous in the rat oocytes and embryos, especially in the ZP. (C) 2007 Elsevier Inc. All rights reserved.