The antioxidant and anticancer properties of a medicinal plant, Romulea tempskyana (R. tempskyana) (Iridaceae) were investigated. The fresh corm water extract of R. tempskyana significantly increased cell viability against H2O2 cytotoxicity. The extract also showed high inhibition of the lipid peroxidation activity (78.20%), reducing power (IC50 64.99 mu g mL(-1)) activity and hydroxyl (IC50 38.66 mu g mL(-1)), superoxide (IC50 25.99 mu g mL(-1)) and DPPH (IC50 19.88 mu g mL(-1)) radical scavenging activity. On the other hand, treatment of the cells with higher extract concentration showed the anticancer activity inducing cytotoxicity. The extract significantly affected Hepatoma G2 and H1299 cell proliferation (IC50; 103.79 and 88.15 mu g mL(-1)). The amount of MDA (2-fold and 2.5-fold) and activities of several cellular antioxidant enzymes, including Se-GSPx (30%, 15%), non-Se-GSH-Px (11%, 16%) and GST (17%, 23%) increased in Hepatoma G2 and H1299 cells treated with IC50 concentrations of extract, respectively. These findings suggest that R. tempskyana extract exhibits antioxidant and carcinogenesis-reducing potential.
The antioxidant and anticancer properties of a medicinal plant, Romulea
tempskyana (R. tempskyana) (Iridaceae) were investigated. The fresh corm
water extract of R. tempskyana significantly increased cell viability against H2O2
cytotoxicity. The extract also showed high inhibition of the lipid peroxidation
activity (78.20%), reducing power (IC50 64.99 mgmL1) activity and hydroxyl
(IC50 38.66 mgmL1), superoxide (IC50 25.99 mgml1) and DPPH (IC50
19.88 mgmL1) radical scavenging activity. On the other hand, treatment of the
cells with higher extract concentration showed the anticancer activity inducing
cytotoxicity. The extract significantly affected Hepatoma G2 and H1299 cell
proliferation (IC50; 103.79 and 88.15 mgmL1). The amount of MDA (2-fold and
2.5-fold) and activities of several cellular antioxidant enzymes, including Se-GSPx
(30%, 15%), non-Se-GSH-Px (11%, 16%) and GST (17%, 23%) increased in
Hepatoma G2 and H1299 cells treated with IC50 concentrations of extract,
respectively. These findings suggest that R. tempskyana extract exhibits antioxidant
and carcinogenesis-reducing potential.
