Population structure and genetic analysis of narrow-clawed crayfish (Astacus leptodactylus) populations in Turkey


AKHAN S., BEKTAŞ Y., BERBER S., KALAYCI G.

GENETICA, cilt.142, sa.5, ss.381-395, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 142 Sayı: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s10709-014-9782-5
  • Dergi Adı: GENETICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.381-395
  • Anahtar Kelimeler: Astacus leptodactylus, Cytochrome oxidase subunit I, Genetic structure, Turkey, FRESH-WATER CRAYFISH, AUSTROPOTAMOBIUS-PALLIPES DECAPODA, MITOCHONDRIAL-DNA, GENUS AUSTROPOTAMOBIUS, CENTRAL ANATOLIA, SPECIES COMPLEX, NOBLE CRAYFISH, ESCHSCHOLTZ, PHYLOGEOGRAPHY, SYSTEMATICS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The genetic differentiation among Turkish populations of the narrow-clawed crayfish was investigated using a partial sequence of cytochrome oxidase subunit I gene (585 bp) of 183 specimens from 17 different crayfish populations. Median joining network and all phylogenetic analyses disclosed a strong haplotype structure with three prominent clades diverged by a range between 20 and 50 mutations and substantial inter-group pairwise sequence divergence (5.19-6.95 %), suggesting the presence of three distinct clades within the Anatolian populations of Astacus leptodactylus. The divergence times among the three clades of Turkish A. leptodactylus are estimated to be 4.96-3.70 Mya using a molecular clock of 1.4 % sequence divergence per million years, pointing to a lower Pliocene separation. The high level of genetic variability (H (d) = 95.8 %, pi = 4.17 %) and numerous private haplotypes suggest the presence of refugial populations in Anatolia unaffected by Pleistocene habitat restrictions. The pattern of genetic variation among Turkish A. leptodactylus populations, therefore, suggests that the unrevealed intraspecific genetic structure is independent of geographic tendency and congruent with the previously reported geographic distribution and number of subspecies (A. l. leptodactylus and A. l. salinus) of A. leptodactylus.