Minimally invasive method for murine brain fixation

Eichenbaum ., Eichenbaum J., Fadiel A., Miller D., Demir N., Naftolin F., ...Daha Fazla

BIOTECHNIQUES, cilt.39, sa.4, ss.487-489, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 4
  • Basım Tarihi: 2005
  • Doi Numarası: 10.2144/000112003
  • Dergi Adı: BIOTECHNIQUES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.487-489
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied.