BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo

Sozen B., DEMİR N., Zernicka-Goetz M.

DEVELOPMENTAL BIOLOGY, cilt.470, ss.84-94, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 470
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.ydbio.2020.11.005
  • Dergi Adı: DEVELOPMENTAL BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.84-94
  • Anahtar Kelimeler: BMP, Extraembryonic ectoderm, Trophoblast stem cells, Embryonic stem cells, CELL SELF-RENEWAL, STEM-CELLS, TROPHOBLAST, DIFFERENTIATION, GASTRULATION, PROLIFERATION, SPECIFICATION, MORPHOGENESIS, ORGANIZATION, SMAD4/DPC4
  • Akdeniz Üniversitesi Adresli: Evet

Özet

At implantation, the mouse embryo undergoes a critical transformation which requires the precise spatiotemporal control of signalling pathways necessary for morphogenesis and developmental progression. The role played by such signalling pathways during this transition are largely unexplored, due to the inaccessibility of the embryo during the implantation when it becomes engulfed by uterine tissues. Genetic studies demonstrate that mutant embryos for BMPs die around gastrulation. Here we have aimed to dissect the role of BMPs during pre-to post-implantation transition by using a protocol permitting the development of the embryo beyond implantation stages in vitro and using stem cells to mimic post-implantation tissue organisation. By assessing both the canonical and non-canonical mechanisms of BMP, we show that the loss of canonical BMP activity compromises the extraembryonic ectoderm development. Our analyses demonstrate that BMP signalling maintains stem cell populations within both embryonic/extra-embryonic tissues during pre-to post-implantation development. These results may provide insight into the role played by BMP signalling in controlling early embryogenesis.