Akademik Veri Yönetim Sistemi
JOURNAL OF MOLECULAR HISTOLOGY, cilt.43, sa.1, ss.107-113, 2012 (SCI-Expanded)
The epithelial cells of the mouse endometrium comprises the recurring physiologic changes such as proliferation and apoptosis induced by the reproductive hormones throughout the estrus cycle. The c-Abl is a protein tyrosine kinase, localized in the different cellular compartments such as nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, interacts with different cellular proteins, including signaling adaptors, kinases, phosphatases, cell-cycle regulators, transcription factors and cytoskeletal proteins. Our hypothesis is that the c-Abl expression in the mouse uterine epithelium shows cyclic changes during the estrus cycle that is involved in regulation of the endometrial epithelial cells. The regulation of c-Abl gene and protein expression in the uterus of intact animals in the different cycle phases was investigated using immunohistochemistry, western blot and semi-quantitative RT-PCR. The immunohistochemistry results revealed that the luminal and the glandular epithelium (LE) and (GE), respectively, showed gradually increase in the expression of c-Abl from the proestrus (P) to the metestrus and followed by a decrease in the diestrus (D). c-Abl immunoreactivity was detected in the both LE and GE cells, especially the LE showed diminished the c-Abl protein expression on the D phase and the minimal value was detected on the P day. The c-Abl protein level in the LE was increased during the M, presenting a high correlation with the hormonal level of this cell type. The result of c-Abl RT-PCR analysis was compatible with pattern of c-Abl protein expression. In conclusion, the stage-specific protein pattern of the mammalian c-Abl tyrosine kinase presented a good correlation with the mouse estrus cycle and may have a regulative function during the uterine remodeling.