Akademik Veri Yönetim Sistemi
EKIN JOURNAL OF CROP BREEDING AND GENETICS, cilt.92, sa.2, ss.141-149, 2023 (Hakemli Dergi)
ABSTRACT
Hatay pepper is preferred in terms of quality characteris tics e.g. hot, fresh and suitable for dried consumption, but it
does not contain any disease resis tance genes. Within the scope, we es tablished the s tudy is aiming to develop resis tant
hybrid varieties to TSWV is causing high yield and quality losses in pepper production areas. For this purpose, s tudies
are carried out to obtain inbreed resis tant lines in a short time, a Tsw gene found in Capsicum chinense, which provides
resis tance to TSWV, was combined with Hatay pepper lines by crossbreeding. Firs t of all, 20 of the Hatay pepper lines
from the gene pool were selected according to weighted grading criteria, then the number was reduced to 4. In C. chinense
lines, molecular screening was performed with SCAC568 primers and individuals with Tsw gene were determined from
donor parents and crosses. After then, backcrosses to Hatay peppers were conducted and BC1F1 was generated. Their
seed samples were sown from them and brought until the firs t true leaves during the transition from seed to seedling
s tage. Meanwhile, molecular analyses were applied to find resis tant individuals with Tsw gene. The homozygous and
heterozygous plants were planted in a greenhouse and used in anther culture s tudy. Whole s tudy takes 22 months from
the initiation of hybridization to the emergence of androgenic embryos and acclimatization to external conditions and
development of DH seeds. Here, these findings are presented as a case s tudy in biotechnology and the combined techniques
are an indispensable part of accelerated breeding processes.