Atıf İçin Kopyala
Akkoyunlu G., Tepekoy F.
Methods in molecular biology (Clifton, N.J.), cilt.1457, ss.269-74, 2016 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
1457
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Basım Tarihi:
2016
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Doi Numarası:
10.1007/978-1-4939-3795-0_20
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Dergi Adı:
Methods in molecular biology (Clifton, N.J.)
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Sayfa Sayıları:
ss.269-74
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Anahtar Kelimeler:
Immunohistochemistry, Mouse ovary, Paraffin sections, Bouin's Fixative, Biotinylated antibodies, ANTIBODY, BIOTIN, TUMORS
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Akdeniz Üniversitesi Adresli:
Evet
Özet
Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme-substrate-chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative.