Potential Role of Poly(ADP-Ribose) Polymerase Activation in the Pathogenesis of Experimental Left Varicocele

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Tekcan M., KÖKSAL İ. T., TAŞATARGİL A., Kutlu O., Gungor E., Celik-Ozenci C.

JOURNAL OF ANDROLOGY, cilt.33, sa.1, ss.122-132, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.2164/jandrol.110.012203
  • Dergi Adı: JOURNAL OF ANDROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.122-132
  • Anahtar Kelimeler: Oxidative stress, PARP, testes, NF-KAPPA-B, RIBOSE POLYMERASE, MALE-INFERTILITY, PART II, RAT, CELLS, APOPTOSIS, CLEAVAGE, PARP-1, INJURY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study was to evaluate whether the poly(adenosine diphosphate[ADP] ribose) polymerase (PARP) pathway is activated by experimental left varicocele. Rats underwent partial ligation of the left renal vein to induce experimental varicocele, and left testes were analyzed 13 weeks after surgery. Tubule degeneration was evaluated by Johnsen score. Expression of 4-hydroxy-2-nonenal (4-HNE) modified proteins, PARP-1, and poly(-ADP-ribose) (PAR) was detected by immunohistochemistry and Western blot. The degree of apoptosis within testes was determined by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling. Light microscopy revealed testicular damage comprising various degrees of seminiferous tubule degeneration. Germ cell apoptotic index and 4-HNE, PAR, and PARP-1 expression in germ cells increased after varicocele induction. Increased oxidative stress and PARP overactivation in testes might be important with regard to impaired testicular function associated with varicocele.

Potential Role of Poly(ADP-Ribose) Polymerase Activation in the Pathogenesis of Experimental Left Varicocele

By:Tekcan, M (Tekcan, Merih); Koksal, IT (Koksal, Ismail Turker)[ 2 ] ; Tasatargil, A (Tasatargil, Arda)[ 3 ] ; Kutlu, O (Kutlu, Omer)[ 4 ] ; Gungor, E (Gungor, Ece); Celik-Ozenci, C (Celik-Ozenci, Ciler)[ 1 ]

 

JOURNAL OF ANDROLOGY

 

Volume: 33

Issue: 1

Pages: 122-132

DOI: 10.2164/jandrol.110.012203

Published: JAN-FEB 2011

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JOURNAL OF ANDROLOGY

JCR® Category Quartile in Category
ANDROLOGY Q2

Data from the 2012 edition of Journal Citation Reports®

Publisher AMER SOC ANDROLOGY, INC, C/O ALLEN PRESS, INC PO BOX 368, LAWRENCE, KS 66044 USA

ISSN: 0196-3635

Research Domain Endocrinology & Metabolism

Abstract

The aim of this study was to evaluate whether the poly(adenosine diphosphate[ADP] ribose) polymerase (PARP) pathway is activated by experimental left varicocele. Rats underwent partial ligation of the left renal vein to induce experimental varicocele, and left testes were analyzed 13 weeks after surgery. Tubule degeneration was evaluated by Johnsen score. Expression of 4-hydroxy-2-nonenal (4-HNE) modified proteins, PARP-1, and poly(-ADP-ribose) (PAR) was detected by immunohistochemistry and Western blot. The degree of apoptosis within testes was determined by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling. Light microscopy revealed testicular damage comprising various degrees of seminiferous tubule degeneration. Germ cell apoptotic index and 4-HNE, PAR, and PARP-1 expression in germ cells increased after varicocele induction. Increased oxidative stress and PARP overactivation in testes might be important with regard to impaired testicular function associated with varicocele.