Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles

ÇOBAN H. B., Demirci A., TURHAN İ.

BIOPROCESS AND BIOSYSTEMS ENGINEERING, cilt.38, sa.8, ss.1431-1436, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 8
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1007/s00449-015-1384-9
  • Dergi Adı: BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1431-1436
  • Anahtar Kelimeler: Phytase, Aspergillus ficuum, Talcum, Fed-batch fermentation, Continuous fermentation, OPTIMIZATION, PHOSPHORUS, MORPHOLOGY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

This study aimed to enhance Aspergillus ficuum phytase production in fed-batch and continuous fermentations with addition of talcum microparticles. Phytase activity almost doubled in fed-batch and continuous fermentations by addition of 15 g/l of talcum compared to the control. Effect of talcum on fungal morphology was also shown that addition of talcum provided smaller fungal pellets and more homogenized fermentation broth compared to the control. Average fungal pellet radius decreased from 500 to 100 A mu m by addition of 15 g/l of talcum in the bioreactors. Also, 15 g/l talcum addition increased phytase productivity and optimum dilution rate in the continuous fermentations from 0.293 to 0.621 U/ml/h and from 0.09 to 0.1/h, respectively, compared to control.