The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients.

Ponti, Giovanni; Maccaferri, Monia; Manfredini, Marco; Kaleci, Shaniko; Mandrioli, Mauro; Pellacani, Giovanni; Ozben, TOMRİS; Depenni, Roberta; Bianchi, Giampaolo; Pirola, Giacomo; Tomasi, Aldo

doi:10.1016/j.cca.2018.01.007

The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients.

Atıf İçin Kopyala

Ponti G., Maccaferri M., Manfredini M., Kaleci S., Mandrioli M., Pellacani G., ...Daha Fazla

Clinica chimica acta; international journal of clinical chemistry, cilt.479, ss.14-19, 2018 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 479
Basım Tarihi: 2018
Doi Numarası: 10.1016/j.cca.2018.01.007
Dergi Adı: Clinica chimica acta; international journal of clinical chemistry
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.14-19
Anahtar Kelimeler: Cell-free fDNA, NanoDrop, Spectrophotometry, Fluorimetry, Qubit, qPCR, Malignant melanoma, Prostate cancer, Laboratory biomarkers, Circulating free tumor DNA, Tumor marker, BLOOD
Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Circulating cell-free tumor DNA (cfDNA) is of crucial interest in oncology. cfDNA constitutes a potential prognostic and therapeutic marker for different solid tumors and can be used in the diagnostic and therapeutic management of cancer patients for which nowadays there are no valid laboratory markers. In the present study, the quality and quantity of the cfDNA were assessed by different quantification procedures, in order to identify the potential applications of these techniques in the preliminary cfDNA quantification.