Protective Effects of Minocycline against Short-Term Ischemia-Reperfusion Injury in Rat Brain

Aras M., Urfali B., Serarslan Y., Ozgur T., ULUTAS K. T., URFALI S., ...Daha Fazla

PEDIATRIC NEUROSURGERY, cilt.49, sa.3, ss.172-178, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 3
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1159/000362202
  • Dergi Adı: PEDIATRIC NEUROSURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.172-178
  • Anahtar Kelimeler: Cerebral ischemia-reperfusion injury, Minocycline, Malondialdehyde, Neuron-specific enolase, Protein S100 beta, FOCAL CEREBRAL-ISCHEMIA, SUPEROXIDE-DISMUTASE, OXIDATIVE STRESS, GLUTATHIONE-PEROXIDASE, HUNTINGTONS-DISEASE, MILD HYPOTHERMIA, ALPHA-TOCOPHEROL, ARTERY OCCLUSION, EMBOLIC STROKE, DAMAGE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study was to assess the effects of minocycline on cerebral ischemia-reperfusion (I/R) injury in rats. The study was carried out on 24 male Wistar albino rats, weighing 200-250 g, which were divided into three groups: (i) control (n = 8), (ii) I/R (n = 8) and (iii) I/R + minocycline (n = 8). Minocycline was administrated at a dose of 90 mg/kg p.o. to the I/R group 48, 24 and 1 h before ischemia. Following bilateral exposure of the common carotid arteries by anterior cervical dissection and separation of the vagus nerve, I/R injury was performed by occlusion. Following reperfusion, malondialdehyde (MDA), superoxide dismutase, glutathione peroxidase and catalase levels in the blood and brain tissue, and creatine kinase (CK), CK-BB, lactate dehydrogenase (LDH), neuron-specific enolase (NSE) and protein S100 beta levels in the blood were measured and the histopathological changes were monitored. Regarding histopathological evaluation, symptoms of degeneration were significantly improved in the I/R + minocycline group compared to the I/R-only group. Statistical analysis of the biochemical parameters revealed significant differences in MDA (p < 0.001), nitric oxide (p < 0.05), CK (p < 0.05) and CK-MB (p < 0.05) levels between the I/R + minocycline group and the I/R group. According to the literature, the effect of minocycline is firstly assessed by LDH, CK-MB, NSE and S-100 beta analysis in addition to antioxidant status and histopathological analysis. (C) 2014 S. Karger AG, Basel