Use of PCR-RFLP analysis of mtDNA cytochrome-b gene to determine genetic differences in Capoeta spp.

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Aksakal E., ERDOĞAN O.

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, cilt.59, sa.4, ss.206-211, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 4
  • Basım Tarihi: 2007
  • Dergi Adı: ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.206-211
  • Anahtar Kelimeler: Capoeta spp., cytochrome-b, mtDNA, PCR, RFLP, MITOCHONDRIAL-DNA, IDENTIFICATION, PRODUCTS, NUCLEAR, TROUT
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

Genetic differences between Capoeta capoeta capoeta, Capoeta capoeta umbla, and Capoeta tinca were determined using PCR-RFLP of mtDNA cytochrome-b (Cyt-b) by amplifying approximately 400-500 bp of this region from each of the three subspecies. The restriction enzymes Spel and Hintl did not indicate genetic differences but Alul and Hpall did. Thus, PCR-RFLP of the mtDNA was used to distinguish between closely related subspecies without having to analyze the entire DNA sequence of specimens. Use of this technique demonstrated that the Cyt-b regions of the three subspecies have different base sequences. The similarity between C. c. capoeta and C. c. umbla is 71.4% while C. tinca is more distant (50% for C. c. umbla and 33.3% for C. c. capoeta). The Cyt-b similarity is consistent with morphological and taxonomical similarities. PCR-RFLP can serve as a tool for genetically identifying subspecies of fish in nature and in aquaculture.