Presence and subcellular localizations of surfactant proteins A and D in human spermatozoa

Kankavi O., Ata A., Celik-Ozenci C., Sati L., Ciftcioglu M. A., Demir R., ...Daha Fazla

FERTILITY AND STERILITY, cilt.90, sa.5, ss.1904-1909, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 90 Sayı: 5
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.fertnstert.2007.09.064
  • Dergi Adı: FERTILITY AND STERILITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1904-1909
  • Anahtar Kelimeler: Surfactant protein A, surfactant protein D, human spermatozoa, Western blot, immunofluorescence, EQUATORIAL SEGMENT, SP-A, HUMAN TISSUES, SPERM, EPIDIDYMIS, GENE, EXPRESSION, BINDING, IDENTIFICATION, EPITHELIUM
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa.

and subcellular localizations of surfactant proteins A and D in human spermatozoa

By:Kankavi, O (Kankavi, Orhan)[ 1 ] ; Ata, A (Ata, Ayhan)[ 2,3 ] ; Celik-Ozenci, C (Celik-Ozenci, Ciler)[ 4 ] ; Sati, L (Sati, Leyla)[ 4 ] ; Ciftcioglu, MA (Ciftcioglu, Mehmet Akif)[ 5 ] ; Demir, R (Demir, Ramazan)[ 4 ] ; Baykara, M (Baykara, Mehmet)[ 6 ]

 

FERTILITY AND STERILITY

 

Volume: 90

Issue: 5

Pages: 1904-1909

DOI: 10.1016/j.fertnstert.2007.09.064

Published: NOV 2008

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FERTILITY AND STERILITY

JCR® Category Quartile in Category
REPRODUCTIVE BIOLOGY Q1
OBSTETRICS & GYNECOLOGY Q1

Data from the 2012 edition of Journal Citation Reports®

Publisher ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA

ISSN: 0015-0282

Research Domain Obstetrics & Gynecology
Reproductive Biology

Abstract

Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa.

Design: Prospective, research study.

Setting: Two universities in Turkey.

Patient(s): Semen specimens (n = 10) were obtained from normozoospermic donors.

Intervention(S): Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein.

Main Outcome Measure(s): Presence of SP-A and SP-D proteins in human beings.

Result(s): Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa.

Conclusion(S): This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa. (Fertil Steril (R) 2008;90:1904-9. (C)2008 by American Society for Reproductive Medicine.)