Akademik Veri Yönetim Sistemi
MolBiyoKon'22 8. International Congress of the Molecular Biology Association of Turkey, İstanbul, Türkiye, 9 - 12 Haziran 2022, ss.139
Abstract
Background/aim: Many herbal compounds are applied for phytotherapy purposes in lung
cancer (H1299), the incidence of which is increasing globally. However, it is a fact that this
compound, which will be used to treat lung cancer, also affects healthy cells. Activation of the
apoptotic pathway was investigated to demonstrate the anticancer effect of catechol on lung
cancer cells. In addition, the activation of the apoptotic pathway was studied with healthy
fibroblast (Bj) cells and the results were compared.
Materials and methods: The 24-hour cytotoxic effect of catechol on cells was demonstrated
by the Cell Titer-Blue® cell viability test. In order to determine the apoptotic potential, the
caspase 3/7 activity of the cells exposed to catechol was measured and the potential of
catechol to stimulate the apoptotic pathway was determined. Caspase 3/7 enzyme activity was
determined using the Promega 'ApoTox-GloTM Triplex Assay kit.
Results: In our study, the IC50 values of the cytotoxic effect of catechol on lung cancer and
healthy fibroblast cells were found to be 58 and 207 μg/ml, respectively. Caspase-3/7 activity
in lung cancer and Bj cells increased 2.8-fold and 1.6-fold, respectively, compared to control
after 24 hours of catechol incubation.
Conclusion: In conclusion, lung cancer cells have higher caspase 3/7 activity than healthy cells,
indicating that catechol has a higher apoptotic potential in cancer cells than in healthy cells.
Keywords: Catechol, Lung cancer, Apoptosis
Acknowledgment : The authors wish to thank Akdeniz University, BAP-Scientific Research
Projects Coordination Unit (FYL-2022-5902) for financial support of this work.