Akademik Veri Yönetim Sistemi
JOURNAL OF ANIMAL AND PLANT SCIENCES, cilt.32, sa.5, ss.1261-1273, 2022 (SCI-Expanded)
C. reinhardtiihas two putative SEC23 genes,CrSEC23AandCrSEC23B. The encoded polypeptides are only ~18.9%identical, suggesting that they might have different functions.It is not clearwhether SEC23 paralogs have same ordifferent functionsin diverse organisms.Interestingly, our alignment and homology modeling showed that CrSEC23Bdoes not have the conserved SEC24 binding motif (VFR), but instead appears to have an LPA motif in the same position.While LPA might be part of a novel SEC24 binding motif,CrSEC23B might have an alternate function that is eitherassociated with or independent of COPII. Our results also show SEC23 orthologs in various organisms have variations inthe putative SEC24 binding motif.Phylogenetic analyses place the SEC23 orthologs into twoclusters that we designatedgroup A (conventional; CrSEC23A-like orthologs) and group B (unconventional; CrSEC23B-like orthologs).Our resultssuggest that many photosynthetic organisms have a divergent SEC23 paralog. This divergence is not seen in animals.Wehypothesizethat divergent (unconventional) SEC23 paralogs might be the result of gene duplication and divergence thatmay facilitate specific aspects of trafficking.Since we only identified the B-like proteins in photosynthetic lineages, wehypothesize that B-like proteins may not have been present in the common ancestor involved in the primaryendosymbiotic event.