Carbon monoxide does not contribute to vascular tonus improvement in exercise-trained rats with chronic nitric oxide synthase inhibition

Ulker S., Kocer G., Senturk U. K.

NITRIC OXIDE-BIOLOGY AND CHEMISTRY, cilt.65, ss.60-67, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.niox.2017.02.011
  • Dergi Adı: NITRIC OXIDE-BIOLOGY AND CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.60-67
  • Anahtar Kelimeler: Carbon monoxide, Hypertension, Exercise, Heme oxygenase, Nitric oxide synthase, SPONTANEOUSLY HYPERTENSIVE-RATS, CHRONIC NOS INHIBITION, BLOOD-PRESSURE, HEME OXYGENASE, VASODILATION, MOLECULES, MODEL, CO
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Carbon monoxide (CO), an end product of heme oxygenase (HO) that is involved in the regulation of vascular tonus, may show a compensatory effect in nitric oxide (NO) deficiency. This study aimed to assess the effect of the HO/CO system on the vascular tone in exercise-trained rats with hypertension induced by chronic NO synthase (NOS) inhibition. Hypertension was induced by N-nitro-L-arginine methyl ester (25 mg/kg/day in drinking water), and exercise training comprised swimming 1h/day, 5 days/week, for 6 weeks. Systolic blood pressure (BP) was measured weekly using a tail-cuff method. The effects of hypertension and/or exercise-training on the constriction and relaxation responses of the thoracic aorta and resistance arteries of the mesenteric and gastrocnemius vascular beds were evaluated. NOS inhibition produced a gradually developed hypertension, and the magnitude of the increase in BP was significantly attenuated by exercise training. Although phenylephrine (Phe)-induced contraction responses of aorta incubated with an HO-1 inhibitor were reduced in hypertensive animals, there was no difference in the hypertensive-exercise group. However, thoracic aortas in the hypertensive-exercise group exhibited significantly more relaxation in response to a CO donor. There was no change in Phe-induced contraction with or without HO inhibition CO donor relaxation responses in both resistance arteries. These results suggest that the HO/CO system does not contribute to diminishing BP by exercise training in a NOS inhibition -induced hypertension model. (C) 2017 Elsevier Inc. All rights reserved.