Antioxidants Induce Apoptosis of Rat Ovarian Theca-Interstitial Cells

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Rzepczynska I. J., Foyouzi N., Piotrowski P. C., Celik-Ozenci C., Cress A., Duleba A. J.

BIOLOGY OF REPRODUCTION, cilt.84, sa.1, ss.162-166, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1095/biolreprod.110.087585
  • Dergi Adı: BIOLOGY OF REPRODUCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.162-166
  • Anahtar Kelimeler: antioxidants apoptosis, ovary, theca interstitial cells, FOLLICLE-STIMULATING-HORMONE, N-ACETYL-CYSTEINE, OXIDATIVE STRESS, LUTEINIZING-HORMONE, CARDIOVASCULAR-DISEASE, REACTIVE OXYGEN, GROWTH-FACTORS, INSULIN, PROLIFERATION, EBSELEN
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Regulation of growth of ovarian theca-interstitial tissues is essential for normal ovarian development and function Reactive oxygen species are involved in modulation of signal transduction pathways, including regulation of tissue growth and apoptosis Previously, we have demonstrated that antioxidants inhibit proliferation of theca-interstitial cells This report evaluates the effects of antioxidants on apoptosis of rat theca-interstitial cells The cells were cultured in chemically defined media without or with vitamin E succinate and ebselen Apoptosis was evaluated by cytochemical assessment of nuclear morphology, activity of executioner caspases 3 and 7, and determination of staining with annexin V in combination with propidium iodide Both tested antioxidants Induced significant morphological changes consistent with apoptosis, including chromatin condensation, nuclear shrinkage, and pyknosis Antioxidants also induced other hallmarks of apoptosis including Increased activity of caspases 3/7 as well as increased staining with annexin V The present findings demonstrate that antioxidants with distinctly different mechanisms of action induce a series of events consistent with the process of apoptosis in ovarian mesenchyme These observations may be of translational-clinical relevance, providing mechanistic support for the use of antioxidants in the treatment of PCOS, a condition associated with excessive growth and activity of theca-interstitial cells

Antioxidants Induce Apoptosis of Rat Ovarian Theca-Interstitial Cells

By:Rzepczynska, IJ (Rzepczynska, Izabela J.)[ 2 ] ; Foyouzi, N (Foyouzi, Nastaran)[ 3 ] ; Piotrowski, PC (Piotrowski, Piotr C.)[ 4 ] ; Celik-Ozenci, C (Celik-Ozenci, Ciler)[ 5 ] ; Cress, A (Cress, Amanda)[ 1 ] ; Duleba, AJ (Duleba, Antoni J.)[ 1 ]

 

BIOLOGY OF REPRODUCTION

 

Volume: 84

Issue: 1

Pages: 162-166

DOI: 10.1095/biolreprod.110.087585

Published: JAN 2011

View Journal Information

BIOLOGY OF REPRODUCTION

JCR® Category Quartile in Category
REPRODUCTIVE BIOLOGY Q1

Data from the 2012 edition of Journal Citation Reports®

Publisher SOC STUDY REPRODUCTION, 1603 MONROE ST, MADISON, WI 53711-2021 USA

ISSN: 0006-3363

Research Domain Reproductive Biology

Abstract

Regulation of growth of ovarian theca-interstitial tissues is essential for normal ovarian development and function Reactive oxygen species are involved in modulation of signal transduction pathways, including regulation of tissue growth and apoptosis Previously, we have demonstrated that antioxidants inhibit proliferation of theca-interstitial cells This report evaluates the effects of antioxidants on apoptosis of rat theca-interstitial cells The cells were cultured in chemically defined media without or with vitamin E succinate and ebselen Apoptosis was evaluated by cytochemical assessment of nuclear morphology, activity of executioner caspases 3 and 7, and determination of staining with annexin V in combination with propidium iodide Both tested antioxidants Induced significant morphological changes consistent with apoptosis, including chromatin condensation, nuclear shrinkage, and pyknosis Antioxidants also induced other hallmarks of apoptosis including Increased activity of caspases 3/7 as well as increased staining with annexin V The present findings demonstrate that antioxidants with distinctly different mechanisms of action induce a series of events consistent with the process of apoptosis in ovarian mesenchyme These observations may be of translational-clinical relevance, providing mechanistic support for the use of antioxidants in the treatment of PCOS, a condition associated with excessive growth and activity of theca-interstitial cells