IDENTIFICATION AND GENETIC DIVERSITY OF MELOIDOGYNE CHITWOODI IN POTATO PRODUCTION AREAS OF TURKEY

Devran Z., Mutlu N., Ozarslandan A., ELEKCİOĞLU İ. H.

NEMATROPICA, cilt.39, sa.1, ss.75-83, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 1
  • Basım Tarihi: 2009
  • Dergi Adı: NEMATROPICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.75-83
  • Anahtar Kelimeler: Columbia root-knot nematode, genetic similarity, identification, Meloidogyne chitwoodi, PCR, RAPD, ROOT-KNOT NEMATODE, HETERODERA-GLYCINES ICHINOHE, RIBOSOMAL INTERGENIC SPACER, AMPLIFIED POLYMORPHIC DNA, MAJOR MELOIDOGYNE, AFLP ANALYSIS, RAPD MARKERS, M-FALLAX, POPULATIONS, VARIABILITY
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

Root-knot nematodes (Meloidogyne spp.) were first detected in Turkey during regional nematode surveys of potato fields of Nigde province. Twelve populations of Meloidogyne were collected and identified based on morphological characteristics as well as molecular methods including species-specific primers, confirming that all twelve populations were M. chitwoodi. To estimate genetic relationships among these twelve populations, random amplified polymorphic DNA (RAPD) analysis was done and the results were subjected to UPGM analysis. Analysis of 394 RAPD markers resulted in a similarity coefficient, ranging between 0.64 and 0.81 with a mean similarity of 0.72. The populations Mc3 and Mc4 displayed the greatest genetic similarity (81%). The lowest level of similarity (65%) was observed between Mc9 and Mc7. The large genetic variation among the M. chitwoodi populations indicates that multiple sources of inoculum might have been introduced to the region or that the populations formerly existed in these potato growing areas of Turkey.