Using CRISPR to understand and manipulate gene regulation

Creative Commons License

Akinci E., Hamilton M. C., Khowpinitchai B., Sherwood R.

DEVELOPMENT, cilt.148, sa.9, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 148 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1242/dev.182667
  • Dergi Adı: DEVELOPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: CRISPR screening, CRISPR-Cas9, Disease modeling, Epigenetics, Gene regulation, PLURIPOTENT STEM-CELLS, TRANSCRIPTION FACTORS, COMPUTATIONAL FRAMEWORK, CELLULAR IDENTITY, CAS9 NUCLEASES, GENOMIC DNA, BETA-CELL, ENHANCER, CHROMATIN, IDENTIFICATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Understanding how genes are expressed in the correct cell types and at the correct level is a key goal of developmental biology research. Gene regulation has traditionally been approached largely through observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic functionalization and gene expression. CRISPR-Cas9 technology has already led to the discovery of new paradigms in gene regulation and, as new CRISPR-based tools and methods continue to be developed, promises to transform our knowledge of the gene regulatory code and our ability to manipulate cell fate. Here, we discuss the current and future application of the emerging CRISPR toolbox toward predicting gene regulatory network behavior, improving stem cell disease modeling, dissecting the epigenetic code, reprogramming cell fate and treating diseases of gene dysregulation.