ETHANOL FERMENTATION FROM MICROWAVE-ASSISTED ACID PRETREATED RAW MATERIALS BY Scheffersomyces stipitis


Germec M., OZCAN A., YILMAZER C., TAS N., ONUK Z., DEMIREL F., ...Daha Fazla

AGROLIFE SCIENTIFIC JOURNAL, cilt.6, sa.1, ss.112-118, 2017 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 1
  • Basım Tarihi: 2017
  • Dergi Adı: AGROLIFE SCIENTIFIC JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.112-118
  • Anahtar Kelimeler: microwave-assisted, pretreatment, detoxification, Scheffersomyces stipitis, shake flask fermentation, ethanol, BIOETHANOL PRODUCTION, PICHIA-STIPITIS, DETOXIFICATION, STRAW
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The production of value-added products from renewable resources have been studied by researchers for years and are still being studied due to the fact that there is a point of interest. In this study, ethanol production from microwave-assisted acid pretreated renewable resources including barley husk, wheat bran, and rye bran was performed. The hydrolysis of renewable resources were performed at 700 W of microwave power, 6.92 min of irradiation time, 1:18.26 w/v of solid-to-liquid ratio, and 3.67% v/v of acid ratio for barley husk, 600 W, 6.92 min, 1: 16.69 w/v, and 1.85% for wheat bran, and 460 W, 6.15 min, 1:17.14 w/v, and 2.72% for rye bran. The hydrolysates were detoxified with 2% (w/v) activated charcoal at 30 degrees C and 150 rpm for 30 min. Then, the enriched hydrolysates were utilized to produce ethanol in shake flask fermentation by Scheffersomyces stipitis (ATCC 58784) that is a xylose fermenting yeast at 150 rpm and 30 degrees C with 5% (v/v) inoculum size. Results indicated that the highest ethanol production (6.15 g/L) was performed in shake flask fermentation with wheat bran medium. However, the lowest ethanol concentration (1.37 g/L) was obtained from barley husk medium. Also, 4.89 g/L of ethanol was produced in rye bran media. Nonetheless, while the highest ethanol yield was calculated to be 44.11% in wheat bran media, the lowest ethanol yield was 12.78% in barley husk medium. On the other hand, the sugar utilization yields in barley husk, wheat bran, and rye bran mediums were also computed to be 85.33, 94.09, and 94.74%, respectively. Consequently, raw materials used in this study can be utilized as good carbon sources for production of ethanol by fermentation.