N-acetylcysteine enhances multidrug resistance-associated protein 1 mediated doxorubicin resistance

Akan I., Akan S., Akça H., Savaş B., Ozben T.

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, cilt.34, sa.10, ss.683-689, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 10
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1111/j.1365-2362.2004.01411.x
  • Dergi Adı: EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.683-689
  • Anahtar Kelimeler: BSO, doxorubicin, GSH, HEK293, MRP1, N-acetylcysteine, ACETYL-L-CYSTEINE, INHIBITS APOPTOSIS, CELL-LINE, MRP GENE, GLUTATHIONE, OVEREXPRESSION, EXPRESSION, SUPPRESSION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background Resistance of cancer cells against anticancer agents is caused partly by multidrug resistance-associated protein 1 (MRP1). The exact mechanism of MRP1-involved multidrug resistance has not yet been clarified, although glutathione (GSH) is likely to have a role for the resistance to occur. N-acetylcysteine (NAC) is a pro-glutathione drug. DL-buthionine (S,R)-sulfoximine (BSO) inhibits GSH synthesis. The aim of our study was to investigate the effect of NAC and BSO on MRP1-mediated doxorubicin resistance in human embryonic kidney (HEK293) and its MRP1-transfected 293MRP cells.