Akademik Veri Yönetim Sistemi
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, cilt.823, ss.588-592, 2018 (SCI-Expanded)
Amperometric biosensors based on alcohol dehydrogenase (ADH) and alcohol oxidase (AOx) were developed for analysis of ethanol, methanol and their mixtures. The first biosensor based on ADH responds only to ethanol, the second biosensor based on AOx responds to both methanol and ethanol. Screen printed carbon electrodes (SPCEs) were used as the base electrodes. They were first modified with nanoparticles (multiwalled carbon nanotubes and gold nanoparticles) and polyneutral red film. Then the enzyme ADH was immobilized on them to prepare ADH based biosensor for ethanol analysis or the enzyme AOx was immobilized on them to prepare AOx based biosensor for methanol analysis. The analytical characterization parameters sensitivity, linear range, limits of detection and quantification were found as 0.432 mu A.mM(-1), 178.5-1000 mu M, 53.5 mu M and 178.5 mu M, respectively, for ethanol analysis with AOx based biosensor, as 0.509 mu A.mM(-1), 335.9-1000 mu M, 100.8 mu M and 335.9 mu M, respectively, for methanol analysis with AOx based biosensor and 0.490 mu A.mM(-1), 320-1000 mu M, 96.1 mu M and 320.2 mu M, respectively for ethanol analysis with ADH based biosensor. The same parameters were also determined for the analysis of different mixtures with AOx based biosensor. The developed biosensors have been tested on a commercial alcoholic drink for the analysis of its ethanol and methanol contents.