Microparticle-enhanced polygalacturonase production by wild type Aspergillus sojae

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Karahalil E., DEMIREL F., Evcan E., Germec M., Tarı C., TURHAN İ.

3 BIOTECH, cilt.7, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s13205-017-1004-2
  • Dergi Adı: 3 BIOTECH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Aluminum oxide, Aspergillus sojae, Morphology engineering, Polygalacturonase, Shake flask fermentation, FICUUM PHYTASE PRODUCTION, SOLID-STATE FERMENTATION, PECTINASE PRODUCTION, CELLULASE PRODUCTION, FUNGAL MORPHOLOGY, PELLET SIZE, FED-BATCH, NIGER, ORYZAE, CULTIVATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Polygalacturonases (PGs), an important industrial enzyme group classified under depolymerases, catalyze the hydrolytic cleavage of the polygalacturonic acid chain through the introduction of water across the oxygen bridge. In order to produce and increase the concentration of this enzyme group in fermentation processes, a new approach called microparticle cultivation, a promising and remarkable method, has been used. The aim of this study was to increase the PG activity of Aspergillus sojae using aluminum oxide (Al2O3) as microparticles in shake flask fermentation medium. Results indicated that the highest PG activity of 34.55 +/- 0.5 U/ml was achieved with the addition of 20 g/L of Al2O3 while the lowest activity of 15.20 +/- 0.2 U/mL was obtained in the presence of 0.1 g/L of Al2O3. In fermentation without microparticles as control, the activity was 15.64 +/- 3.3 U/mL. Results showed that the maximum PG activity was 2.2-fold higher than control. Additionally, smaller pellets formed with the addition of Al2O3 where the lowest pellet diameter was 955.1 lm when 10 g/L of the microparticle was used. Also, it was noticed that biomass concentration gradually increased with increasing microparticle concentration in the fermentation media. Consequently, the PG activity was significantly increased in microparticle-enhanced shake flask fermentation. In fact, these promising preliminary data can be of significance to improve the enzyme activity in large-scale bioreactors.