Akademik Veri Yönetim Sistemi
8th International Congress of the Molecular Biology Association of Turkey (MolBiyokon) , İstanbul, Türkiye, 9 - 12 Haziran 2022, ss.159-160
Background/aim: In recent years, attention has been drawn to the increasing incidence of skin
cancer. As a result, interest in higher plants with antioxidant capacity is increasing, as they can
delay the progression of many chronic diseases. The cytotoxic effect of Origanum majorana
essential oil on epidermoid carcinoma (A431) cells was measured by two different viability
tests and the results were compared.
Materials and methods: A431 (Epidermoid Carcinoma) cell line was obtained from the
Molecular Biology and Genetics Laboratory of Akdeniz University, Faculty of Science, in
frozen form. Cells were routinely cultured in Dulbeccos Modified Eagles Medium (DMEM),
supplemented with 10% fetal calf serum, and 1% antibiotic-antimycotic solution in atmosphere
containing 5% CO2 at 37 C. For subculturing, cells were harvested after a trypsin/EDTA
treatment at 37 °C and plated into 96-well plates. The 24-hour cytotoxic effect of Origanum
majorana essential oil on cells were demonstrated by the Cell Titer-Blue® cell viability and
MTT tests.
Results: In our study, the IC50 values of the cytotoxic effect of Origanum majorana essential
oil on epidermoid carcinoma (A431) cells with MTT and Cell Titer-Blue® cell viability test
were found to be 75 μg/ml and 74 μg/ml respectively.
Conclusion: This study showed that the essential oil of Origanum majorana exhibited a
cytotoxic effect due to the increased concentration. The results of both cytotoxic tests measuring
viability are very close to each other, confirming the results.
Keywords: Origanum majorana (L.), Skin cancer, Cytotoxic
Acknowledgement : The author wish to thanks to Molecular Biology and Genetics
Laboratory of Akdeniz University/Faculty of Science for support of this work.