Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Türkiye
Tezin Onay Tarihi: 2018
Tezin Dili: Türkçe
Öğrenci: Nuray Sarı
Asıl Danışman (Eş Danışmanlı Tezler İçin): Hakan Fidan
Özet:
Tomato Spotted Wilt Virus (TSWV) is one
of the plant viruses that spreads rapidly with thrips vectors and causes the
most common and economically importantyield losses in worldwide.Sw-5gene in tomato and Tsw genes in pepper are responsible for
the resistance towards TSWV. However, it has recently been reported that
resistance to these genes broken allowing spreads of TSWV infections on tomato varieties
with Sw-5 and Tsw resistance genes in many parts of the World.The main purpose of
this study was to understand the mechanism of the breaking of TSWV resistance
in tomato varieties with the resistance genes in Turkey.The resistance-breaking
TSWV isolate (TSWV AntRB) was collected and its whole genom sequences were
compared with those of the World’s TSWV isolates in order to establish
phylogenetic trees and find out a mutation which breaks the Sw-5 gene resistance. Based on the whole
genome sequences, the TSWV genome has consistedof three-part segmented
structures which were: Large segments-8913 nt, Medium-4752 nt and Small-2924 nt
length regions. Their three regions coding proteins were amplified by RT-PCR,
and their sequences were obtained. Open reading regions responsible for protein
synthesis were identified on the gene sequences. Differences in nucleotides,
amino acid and protein levels were revealed in these regions. There was a
mutation on non-structural protein (NSm) region. The NSm region is responsible
cell to cell movement where a mutation at the C118Y site on the NSm domain has
been determined. These mutations allow breaking of resistance. Additionally, the
comparisonsof the M and S segments(TSWVAntRB) have showed 99% similarity with those
of the virus isolates broken resistance in Spain. It has been determined that analysis
located the virus on the same root and same branch in the same place. The phylogentic
trees have shown us that the isolate TSWVAntRB has come from the same origin in
Europe.